OBSERVATIONS ON FIXATION AND STAINING OF BONE-MARROW BIOPSIES

Ten bone marrow (BM) biopsies were taken from the iliac crest of six autopsy cases. The tissues were fixed in Zenker''s fixative for intervals between one and 18 hours with and without decalcification. Ultrasonic bombardment was used for one and two hours. The same procedures were also used following 18 hours fixation in 10% neutral buffered formalin. Coded slides were double blind evaluated for section quality and cytologic detail after staining with H and E, three Giemsa variants, and Gomori''s iron stain. Paraffin processed BM biopsies were optimally prepared by fixation in Zenker''s fluid for 4-18 hours without decalcification. Prior formalin fixation did not influence the consistent brilliance of Giemsa staining with the preferred Sheehan Wright Giemsa stain. Overnight Zenker fixation with or without prior formalin exposure and the absence of decalcification is recommended for the routine preparation of BM biopsies for paraffin processing.