RHEUMATOID-FACTOR QUANTITATION - A COMPARISON OF ELISA AND NEPHELOMETRIC METHODS

Quantitation of rheumatoid factors (RF) by nephelometry, and more recently by enzyme linked immunosorbent assay (ELISA), are achieving increasingly common usage. Sera from 101 rheumatoid patients were compared by both methods and screened by a latex fixation method. Good correlation was found between the two assay techniques, the ELISA showing greater sensitivity and measuring IgG RF and IgA RF in addition to IgM RF. Both assay methods were reliable, accurate, rapid and sensitive for routine clinical purposes.