NUCLEOTIDE-SEQUENCE AND CHARACTERIZATION OF THE FELINE HERPESVIRUS TYPE-1 IMMEDIATE-EARLY GENE

We report the nucleotide sequence of feline herpesvirus type 1 (FHV-1) immediate early (IE) gene encoding a homologue to the ICP4 protein of herpes simplex virus type 1 and its flanking sequences. When we examined the regulatory function of the FHV-1 IE gene product by using the transient transfection assay with an effector plasmid expressing the FHV-1 IE gene in combination with chimeric various promoter-chloramphenicol acetyltransferase reporter constructs, the product was able to transactivate a putative FHV-1 TK promoter, pseudorabies virus and equine herpesvirus type 1 ICP4 promoters, and human immunodeficiency virus (HIV) long terminal repeat (LTR) in Crandell feline kidney cells. Furthermore, the FHV-1 IE gene product could transactivate both feline immunodeficiency virus and HIV LTRs in SW480 cells but not in Felis catus whole fetus 4 cells. (C) 1994 Academic Press, Inc.