IMPROVED METHODOLOGY FOR THE QUANTITATIVE ASSESSMENT OF TOBACCO-SPECIFIC N-NITROSAMINES IN TOBACCO BY SUPERCRITICAL-FLUID EXTRACTION

被引：28

作者：

PROKOPCZYK, B ^{[1
]}

WU, M ^{[1
]}

COX, JE ^{[1
]}

AMIN, S ^{[1
]}

DESAI, D ^{[1
]}

IDRIS, AM ^{[1
]}

HOFFMANN, D ^{[1
]}

机构：

[1] UNIV KHARTOUM,FAC DENT,DEPT ORAL SURG,KHARTOUM,SUDAN

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1995年 / 43卷 / 04期

关键词：

TOBACCO-SPECIFIC N-NITROSAMINES; TSNA; SUPERCRITICAL FLUID EXTRACTION; SFE;

D O I：

10.1021/jf00052a013

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

This paper describes an analytical method for the assessment of tobacco-specific N-nitrosamines (TSNA) in tobacco which involves supercritical fluid extraction and quantification by gas chromatography with thermal energy analyzer detection. Extraction of tobacco with supercritical carbon dioxide, to which 10% methanol is added, is followed by a cleanup on silica cartridges which separates the TSNA from the bulk of other tobacco components present in the extract. Gas chromatographic separation is performed on a DB-5 fused silica capillary column, and quantitative results are ascertained by using 4-(ethylnitrosamino)-1-(3-pyridyl)-1-butanone as an internal standard. This method is quantitative and well reproducible and meets the qualifications to be chosen as a standardized analytical method.

引用

页码：916 / 922

页数：7

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