POLYMORPHISM OF THE HUMAN CD46 GENE IN NORMAL INDIVIDUALS AND IN RECURRENT SPONTANEOUS-ABORTION

被引：37
|
作者：
RISK, JM [1 ]
FLANAGAN, BF [1 ]
JOHNSON, PM [1 ]
机构：
[1] UNIV LIVERPOOL,DEPT IMMUNOL,POB 147,LIVERPOOL L69 3BX,ENGLAND
来源：
HUMAN IMMUNOLOGY | 1991年 / 30卷 / 03期
关键词：
D O I：
10.1016/0198-8859(91)90030-D
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Restriction fragment length polymorphism analysis of the human CD46 gene, which encodes a cell surface complement regulatory protein, has been performed using a 1.5-kb cDNA probe containing a complete CD46 coding sequence. The sum of the invariant band sizes indicates that this gene encompasses at least 35-kb genomic DNA. Polymorphisms were detected in normal individuals with both HindIII and EcoRI. Several of the polymorphic bands occurred significantly less frequently in recurrent spontaneous abortion individuals. Northern blot analysis has shown the predominant RNA transcript size to be 4.2 kb in trophoblast-derived cell lines and peripheral blood mononuclear cells, indicating these transcripts may contain an unusually long 3'-untranslated region.
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页码：162 / 167
页数：6
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