THE CDC8 GENE-PRODUCT IS REQUIRED FOR TRANSFORMATION WITH EPISOMAL AND INTEGRATIVE PLASMIDS IN SACCHAROMYCES-CEREVISIAE

被引：2
|
作者：
LECKACZERNIK, B
ZUK, J
机构：
[1] Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, PL-02-532
来源：
CURRENT GENETICS | 1991年 / 20卷 / 04期
关键词：
CDC8; TRANSFORMATION; REPLICATION; SACCHAROMYCES-CEREVISIAE;
D O I：
10.1007/BF00318513
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The product of the yeast CDC8 gene (thymidylate kinase), which is required for chromosomal, mitochondrial and 2-mu plasmid replication, also participates in plasmid transformation processes in S. cerevisiae. The thermosensitive cdc8-1 mutant strain was transformed with episomal pDQ9 and integrative pDQ9-1 plasmids both of which carry the CDC8 gene. The results suggest that thymidylate kinase is essential for the expression of genes carried on transforming episomal plasmid DNA (probably through its replication) and is also essential for homologous recombination between chromosomal and linearized integrative plasmid DNA.
引用
收藏
页码：265 / 267
页数：3
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