DEVELOPMENT OF AN ASSAY-METHOD FOR PURINE CATABOLIC ENZYMES IN THE MOUSE AND ITS ADAPTATION FOR USE ON AN AUTOANALYZER

被引：9
|
作者：
LETISSIER, PR
PETERS, J
SKIDMORE, CJ
机构：
[1] UNIV READING, DEPT BIOCHEM & PHYSIOL, POB 228, READING RG6 2AJ, BERKS, ENGLAND
[2] MRC, RADIOBIOL UNIT, DIDCOT OX11 0RD, OXON, ENGLAND
来源：
ANALYTICAL BIOCHEMISTRY | 1994年 / 222卷 / 01期
基金：
英国医学研究理事会;
关键词：
D O I：
10.1006/abio.1994.1469
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An assay method has been developed for the purine catabolic enzymes adenosylhomocysteinase, adenosine deaminase (ADA), purine-nucleoside phosphorylase (PNP), and urate oxidase in mice. The assay links H2O2 produced during purine catabolism to the production of a dye complex. The assay method has been developed for ADA and PNP in erythrocytes and for all four enzymes in liver. The assay is cheap, sensitive, and easy to perform. The dye complex absorbs in the visible range, negating the need for an expensive ultraviolet spectro-photometer and allowing the use of an autoanalyzer. (C) 1994 Academic Press, Inc.
引用
收藏
页码：168 / 175
页数：8
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