DNA DOUBLE-STRAND BREAK ANALYSIS BY CHEF ELECTROPHORESIS

被引：46
|
作者：
BLOCHER, D
KUNHI, M
机构：
[1] Biomedical Physics Department, King Faisal Specialist Hospital and Research Centre, POB 3354, Riyadh
来源：
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY | 1990年 / 58卷 / 01期
关键词：
D O I：
10.1080/09553009014551411
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Pulsed-field gel electrophoresis (PFGE) has become a powerful tool for the study of high-molecular-weight DNA. In this technical note the application of clamped homogeneous electrical field (CHEF) electrophoresis for the determination of DNA double-strand breaks is described, and a number of parameters that influence the sensitivity of the assay are discussed. They include the concentration of the cell sample, agarose concentration in the plug and the electrophoresis gel, lysis conditions, pulse time, electric field gradient and the temperature of electrophoresis. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
引用
收藏
页码：23 / 34
页数：12
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