AMPHIBIAN INTESTINAL BRUSH-BORDER MEMBRANES .1. ISOLATION FROM RANA-CATESBEIANA TADPOLE

被引：12
|
作者：
DAUCA, M
HOURDRY, J
HUGON, JS
MENARD, D
机构：
[1] UNIV SHERBROOKE, FAC MED, DEPT ANAT & BIOL CELLULAIRE, UNITE BIOL INTESTINALE, SHERBROOKE J1H 5N4, QUEBEC, CANADA
[2] UNIV PARIS 11, CTR ORSAY, BIOL VERTEBRES LAB, F-91405 ORSAY, FRANCE
[3] ECOLE NORM SUPER, BIOL & BIOCHIM DEV LAB, F-92211 ST CLOUD, FRANCE
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1979年 / 64卷 / 02期
关键词：
D O I：
10.1016/0305-0491(79)90154-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Intestinal brush border membrane vesicles were isolated from R. catesbeiana tadpole. EM of brush border membrane vesicles demonstrates a fairly homogenous preparation of vesicles, some of them still containing electron dense material. The dense vesicles probably comprise both microvillus core and membrane. Negative staining of vesicles reveals the presence of knob-like structures (particles) covering the outer surface of the membrane. The membranous fraction is characterized by a high specific activity of alkaline phosphatase, trehalase, glucoamylase, maltase and .gamma.-glutamyltranspeptidase.
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页码：155 / 159
页数：5
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