EFFECT OF LIPIDS AND DETERGENTS ON THE PRODUCTION AND SOLUBILIZATION OF EXTRACELLULAR LIPASE IN YARROWIA-LIPOLYTICA

Triglycerides, oleic acid but not fatty acid-containing, nonionic detergents (Spans, Tweens) were able to stimulate the synthesis of cell-bound and soluble lipase of Yarrowia lipolytica grown in a complex medium containing cirate and urea. The optimal concentration of olive oil for induction was 0.5 % (W/V). The combined effect of a high ionic strength (0.75 mol/L KCl) and of digitonin (2 mmol/L) at pH 7.6 resulted in solubilization of 80 % of the cell-bound lipase and a significant activation of the enzyme. Comparison of two Y. lipolytica strains showed the effects of Mg2+ and Fe3+ concentrations in the medium on the synthesis of the enzyme to be strongly strain-dependent.