EFFECTS OF 20-HYDROXYECDYSONE ON PROTEIN-SYNTHESIS IN THE EPITHELIAL-CELL LINE FROM CHIRONOMUS-TENTANS

被引：7

作者：

FRETZ, A ^{[1
]}

LUBBERT, A ^{[1
]}

MARKMANNMULISCH, U ^{[1
]}

SPINDLERBARTH, M ^{[1
]}

SPINDLER, KD ^{[1
]}

机构：

[1] HEINRICH HEINE UNIV DUSSELDORF,INST ZOOL,UNIV STR,W-4000 DUSSELDORF 1,GERMANY

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1993年 / 23卷 / 01期

关键词：

PROTEIN SYNTHESIS; TRANSCRIPTION; 20-OH-ECDYSONE; CHIRONOMUS; INSECT; CELL LINE; MOLTING HORMONE;

D O I：

10.1016/0965-1748(93)90095-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the epithelial cell line of Chironomus tentans growth ceases after addition of 20-hydroxyecdysone. Under the influence of moulting hormone the protein pattern changes as shown by 2-dimensional electrophoresis and silver staining. Incorporation of S-35-labelled methionine reveals qualitative and quantitative changes in the homogenate from the cells and in the proteins secreted into the medium. RNA from cells treated with 20-OH-ecdysone for various times either permanently or with pulses of moulting hormones was expressed in a reticulocyte in vitro translation system. Qualitative changes in the in vitro translated products were first observed after 75 min and continue until day 4 after hormone treatment. A complex pattern of permanent and transient induction and repression of different transcripts is obtained.

引用

页码：159 / 164

页数：6

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