AN ELISA FOR ANTIBODIES TO RECOMBINANT GLUTAMIC-ACID DECARBOXYLASE IN IDDM

被引:52
|
作者
DEAIZPURUA, HJ
HARRISON, LC
CRAM, DS
机构
关键词
D O I
10.2337/diabetes.41.9.1182
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
To detect serum antibodies to GAD in subjects with IDDM, three recombinant mBGAD 67 peptides encompassing the full-length protein were used in an ELISA. In this study 7 of 9 (78%) preclinical IDDM subjects (ICA+ first-degree relatives of a person with IDDM) and 6 of 13 (46%) recent-onset IDDM subjects, but no subjects with Graves' disease (n = 10) or scleroderma (n = 10), nor healthy nondiabetic control subjects (n = 10) had antibodies that reacted with one or more of the recombinant mBGAD peptides. We found no preferential reactivity with any recombinant peptide. Although only 3 preclinical subjects and 1 recent-onset subject had antibodies to all three mBGAD peptides, the results indicate that mBGAD 67 contains at least three B-cell autoepitopes. Compared with an immunoprecipitation assay of native human brain GAD, the ELISA detected 5 of 6 (83%) preclinical and 6 of 6 (100%) recent-onset IDDM subjects. The ELISA should facilitate screening to evaluate the role of autoimmunity to GAD in the development of IDDM.
引用
收藏
页码:1182 / 1187
页数:6
相关论文
共 50 条
  • [31] RADIOCHEMICAL ASSAY FOR GLUTAMINASE AND GLUTAMIC-ACID DECARBOXYLASE
    WOOD, AW
    SEEGMILL.JE
    MCCREA, ME
    ANALYTICAL BIOCHEMISTRY, 1972, 48 (02) : 581 - &
  • [32] GLUTAMIC-ACID DECARBOXYLASE EXPRESSION IN ISLETS AND BRAIN
    GIORDA, R
    PEAKMAN, M
    TAN, KC
    VERGANI, D
    TRUCCO, M
    LANCET, 1991, 338 (8780): : 1469 - 1470
  • [33] PREDICTIVE VALUE OF ANTIBODIES TO GLUTAMIC-ACID DECARBOXYLASE (ANTI-GAD) FOR INSULIN REQUIREMENTS
    TSURUOKA, A
    MATSUBA, I
    NEMOTO, M
    IKEDA, Y
    DIABETOLOGIA, 1993, 36 : A98 - A98
  • [34] GLUTAMIC-ACID DECARBOXYLASE, GLUTAMINE-SYNTHETASE AND GLUTAMIC-ACID DEHYDROGENASE IN VARIOUS AREAS OF HUMAN BRAIN
    VOGEL, WH
    HEGINBOTHOM, SD
    BOEHME, DH
    BRAIN RESEARCH, 1975, 88 (01) : 131 - 135
  • [35] PREVALENCE OF ANTIBODIES TO GLUTAMIC-ACID DECARBOXYLASE IN WOMEN WHO HAVE HAD GESTATIONAL DIABETES
    BEISCHER, NA
    WEIN, P
    SHEEDY, MT
    MACKAY, IR
    ROWLEY, MJ
    ZIMMET, P
    AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1995, 173 (05) : 1563 - 1569
  • [36] MONOCLONAL-ANTIBODIES AGAINST RAT-BRAIN GLUTAMIC-ACID DECARBOXYLASE (GAD)
    LEGAY, F
    HENRY, S
    TAPPAZ, ML
    NEUROCHEMISTRY INTERNATIONAL, 1987, 10 (03) : 287 - 294
  • [38] HIGH PREVALENCE OF AUTOANTIBODIES TO GLUTAMIC-ACID DECARBOXYLASE IN LONG-STANDING IDDM IS NOT A MARKER OF SYMPTOMATIC AUTONOMIC NEUROPATHY
    ZANONE, MM
    PETERSEN, JS
    PEAKMAN, M
    MATHIAS, CJ
    WATKINS, PJ
    DYRBERG, T
    VERGANI, D
    DIABETES, 1994, 43 (09) : 1146 - 1151
  • [39] RECOMBINANT GLUTAMIC-ACID DECARBOXYLASE (REPRESENTING THE SINGLE ISOFORM EXPRESSED IN HUMAN ISLETS) DETECTS IDDM-ASSOCIATED 64,000-MR AUTOANTIBODIES
    KARLSEN, AE
    HAGOPIAN, WA
    PETERSEN, JS
    BOEL, E
    DYRBERG, T
    GRUBIN, CE
    MICHELSEN, BK
    MADSEN, OD
    LERNMARK, A
    DIABETES, 1992, 41 (10) : 1355 - 1359
  • [40] SERUM GLUTAMIC-ACID DECARBOXYLASE ACTIVITY IN NORMAL DOGS
    AYERS, JL
    RAMSEY, FK
    VREEKEN, E
    JOWETT, D
    AMERICAN JOURNAL OF VETERINARY RESEARCH, 1978, 39 (11) : 1802 - 1804