PRODUCTION OF ENKEPHALIN IN TOBACCO PROTOPLASTS USING TOBACCO MOSAIC-VIRUS RNA VECTOR

被引：58
|
作者：
TAKAMATSU, N
WATANABE, Y
YANAGI, H
MESHI, T
SHIBA, T
OKADA, Y
机构：
[1] SUMITOMO CHEM CO LTD,TAKARAZUKA RES CTR,BIOTECHNOL LAB,TAKARAZUKA,HYOGO 665,JAPAN
[2] UNIV TOKYO,FAC SCI,DEPT BIOPHYS & BIOCHEM,TOKYO 113,JAPAN
来源：
FEBS LETTERS | 1990年 / 269卷 / 01期
关键词：
Enkephalin; Fusion protein; RNA vector; Tobacco mosaic virus; Transcription system; in vitro;
D O I：
10.1016/0014-5793(90)81121-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To examine the validity of the strategy to express a foreign gene as a fusion protein with the coat protein (CP) of tobacco mosaic virus (TMV), we have constructed ENK RNA by using an in vitro transcription system of TMV RNA. ENK RNA differs from TMV RNA only in that ENK RNA carries an additional sequence coding for Leu-enkephalin (Tyr-Gly-Gly-Phe-Leu) (Enk) with a preceding in-frame methionine just before the termination codon of CP gene. In protoplasts inoculated with ENK RNA, CP+Enk fusion protein accumulated as the major protein. © 1990.
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页码：73 / 76
页数：4
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