ADSORPTION OF CELLULASES ON STEAM-PRETREATED WILLOW

被引：9
|
作者：
GALBE, M [1 ]
EKLUND, R [1 ]
ZACCHI, G [1 ]
机构：
[1] UNIV LUND, POB 124, S-22100 LUND, SWEDEN
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1990年 / 24-5卷
关键词：
adsorption; cellulases; enzyme recovery; willow;
D O I：
10.1007/BF02920236
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The adsorption of cellulases on steam-pretreated willow has been measured for 2, 4, and 8 wt% willow and with varying enzyme concentrations (2-100 wt% based on substrate). The enzyme concentration was measured as soluble protein, filter paper activity (FPA), CMC-ase activity, and activity toward willow. The adsorption data were modeled with a Langmuir isotherm. The maximum adsorption capability depends on the method for measurement of the enzyme concentration. The lowest value, 470 mg enzyme/g willow, was obtained for the soluble protein and the highest value, 650 mg/g, for the FPA. For technical applications, a single isotherm can be used for calculation of the adsorption capability of steam-treated willow. © 1990 Humana Press Inc.
引用
收藏
页码：87 / 101
页数：15
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