BCOAI, A NEW SITE-SPECIFIC ENDONUCLEASE FROM BACILLUS-COAGULANS

被引：0

作者：

SOKOLOV, NN ^{[1
]}

ELDAROV, MA ^{[1
]}

ANIKEITCHEVA, NV ^{[1
]}

KARPYCHEV, IV ^{[1
]}

SAMKO, OT ^{[1
]}

FITZNER, AB ^{[1
]}

KALUGIN, AA ^{[1
]}

CHOROSHOUTINA, EB ^{[1
]}

SKRYABIN, KG ^{[1
]}

机构：

[1] VA ENGELHARDT MOLEC BIOL INST,CTR BIOENGN,MOSCOW,USSR

来源：

BIOORGANICHESKAYA KHIMIYA | 1991年 / 17卷 / 09期

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学科分类号：

摘要：

A new site-specific endonuclease was detected in toluene lysates of Bacillus coagulans AUCM B-732 and designated as BcoAI. The enzyme was purified by fractionation of the cell-free extract in the two-phase PEG/dextran system followed by chromatography on DEAE-sepharose and phosphocellulose and shown to be free of nonspecific nucleases and phosphatases. BcoAI has three cleavage sites on lambda-DNA, but does not cleave SV40, pBR322 and pUC19 DNA. BcoAI recognizes the sequence 5' CAC down GTG 3' on double-stranded DNA and cleaves it as indicated by the arrow to yield blunt-ended DNA fragments. Thus, BcoAI is a true isoschizomer of PmaCI from Pseudomonas maltophila C.

引用

页码：1188 / 1192

页数：5

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