LEISHMANIA-SPP - EFFECT OF INHIBITORS ON GROWTH AND ON POLYAMINE AND MACROMOLECULAR SYNTHESES

被引：35

作者：

BACHRACH, U ^{[1
]}

BREM, S ^{[1
]}

WERTMAN, SB ^{[1
]}

SCHNUR, LF ^{[1
]}

GREENBLATT, CL ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM,HADASSAH HOSP & MED SCH,CTR STUDY INFECT & TROP DIS,DEPT PROTOZOOL,JERUSALEM,ISRAEL

来源：

EXPERIMENTAL PARASITOLOGY | 1979年 / 48卷 / 03期

关键词：

Culture; Ethidium bromide; Growth cycle; Hemoflagellates; Inhibitors; Leishmania donovani Protozoa; parasitic; Leishmania mexicana; Leishmania spp; Leishmania tropica major; MGBG [methylglyoxal-bis (guanylhydrazone); Pentamidine; Polyamines; Putrescine; Spermidine; Spermine;

D O I：

10.1016/0014-4894(79)90131-0

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Ethidium bromide, pentamidine isethionate, and MGBG [methylglyoxal-bis (guanylhydrazone)] inhibited the uptake of radioactive putrescine by leishmanial (Leishmania spp.; Leishmania tropica major; Leishmania mexicana; Leishmania donovani) promastigotes and interfered with their polyamine synthesis. Inhibition was apparent as early as 1 hr after adding these drugs to the parasites at growth-inhibiting concentrations. Ethidium bromide also inhibited the incorporation of radioactive uracil into leishmanial RNA at growth-inhibiting concentrations, while DNA synthesis was inhibited by ethidium bromide at high concentrations after a lag period. MGBG inhibited the synthesis of leishmanial DNA and RNA at growth-inhibiting concentrations. © 1979.

引用

页码：464 / 470

页数：7

共 14 条

[1] THERAPY OF LEISHMANIASIS - SUPERIOR EFFICACIES OF LIPOSOME-ENCAPSULATED DRUGS [J].