LEISHMANIA-SPP - CELLULAR-LEVELS AND SYNTHESIS OF POLYAMINES DURING GROWTH CYCLES

被引：28

作者：

BACHRACH, U ^{[1
]}

BREM, S ^{[1
]}

WERTMAN, SB ^{[1
]}

SCHNUR, LF ^{[1
]}

GREENBLATT, CL ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM,HADASSAH HOSP & MED SCH,CTR STUDY INFECT & TROP DIS,DEPT PROTOZOOL,JERUSALEM,ISRAEL

来源：

EXPERIMENTAL PARASITOLOGY | 1979年 / 48卷 / 03期

关键词：

Culture; Growth cycle; Hemoflagellates; Leishmania donovani; Leishmania mexicana; Leishmania spp; Leishmania tropica major; Polyamines; Protozoa; parasitic; Putrescine; Spermidine; Spermine;

D O I：

10.1016/0014-4894(79)90130-9

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Polyamines were extracted from five different leishmanial strains (Leishmania sp., L. tropica major, L. mexicana, and two L. donovani isolates) and identified as pulrescine, spermidine, and spermine by thin-layer chromatography and mass spectrometry. These sensitive methods were also used to demonstrate the conversion of radioactive putrescine into spermidine and spermine. As in other types of cells, polyamine levels fluctuated during the growth cycle, maximal levels being attained during the logarithmic growth phase. In the five leishmanial strains, which were members of four different serotypes, the spermidine-putrescine ratios also varied, and in two strains of the same serotype, polyamine ratios were practically identical, suggesting that polyamine characteristics might serve as a further criterion for strain identification and classification. © 1979.

引用

页码：457 / 463

页数：7

共 20 条

[1]

ADLER S., 1926, ANN TROP MED & PARASITOL, V20, P355

[2]

Bacchi C. J., 1978, Advances in Polyamine Research, V2, P129

[3] POLYAMINES IN TRYPANOSOMATIDS [J].