PRACTICAL METHOD FOR ESTIMATING OXYGEN KINETIC AND METABOLIC PARAMETERS

被引：14
|
作者：
CHO, MH [1 ]
WANG, SS [1 ]
机构：
[1] RUTGERS STATE UNIV,DEPT CHEM & BIOCHEM ENGN,PISCATAWAY,NJ 08854
来源：
BIOTECHNOLOGY PROGRESS | 1990年 / 6卷 / 02期
关键词：
D O I：
10.1021/bp00002a012
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A practical and simple method was developed to determine the oxygen kinetic and metabolic parameters of cell cultures. The said parameters are the oxygen kinetic constant (Ko2), the maximum specific oxygen uptake rate (q o 2,maxmax), the maximum oxygen yield (Y o 2,max), the critical dissolved oxygen (DO) (Ccr), and the oxygen maintenance coefficient (mo2). These parameters can be calculated from the results of one batch fermentation run. The fermentation needs to be run at an oxygen‐limiting condition, and the profiles of DO and cell density need to be recorded. A demonstration of the use of this method was done by using a yeast, Kluyveromyces marxianus. Copyright © 1990 American Institute of Chemical Engineers (AIChE)
引用
收藏
页码：164 / 167
页数：4
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