New molecular biology methods for protein engineering

This review outlines recent advances in the application of molecular biological techniques to the study of protein structure and function. The review is divided into four main sections: methods for oligonucleotide-directed mutagenesis; mutational strategies for identifying functional residues and domains; systems for expression; and, future developments. Few new methods were reported in 1990; however, a number of the papers that appeared represent refinements of previously reported strategies.