CA2+ BINDING OF LATENT TRANSFORMING GROWTH-FACTOR-BETA-1 BINDING-PROTEIN

被引：22
|
作者：
COLOSETTI, P
HELLMAN, U
HELDIN, CH
MIYAZONO, K
机构：
[1] Ludwig Institute/or Cancer Research, Biomedical Center, S-751 24 Uppsala
来源：
FEBS LETTERS | 1993年 / 320卷 / 02期
关键词：
TRANSFORMING GROWTH FACTOR-BETA; BINDING PROTEIN; EGF-LIKE REPEAT; CA2+ BINDING;
D O I：
10.1016/0014-5793(93)80079-A
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Latent transforming growth factor-beta1 binding protein (LTBP) is a constituent of the latent high molecular weight complex of TGF-beta1 in human platelets. In the present communication, we show that LTBP binds Ca2+ in its free form as well as in the latent TGF-beta1 complex. The binding of Ca2+ induces a structural change which protects the molecule against proteolysis and changes its elution position when analyzed by anion exchange chromatography. The in vitro activation of TGF-beta1 is not influenced by the presence of Ca2+. The possible significance of Ca2+ binding of LTBP is discussed.
引用
收藏
页码：140 / 144
页数：5
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