HUMAN MITOCHONDRIAL ATP SYNTHASE - CLONING CDNA FOR THE NUCLEAR-ENCODED PRECURSOR OF COUPLING FACTOR-6

被引:6
|
作者
JAVED, AA [1 ]
OGATA, K [1 ]
SANADI, DR [1 ]
机构
[1] BOSTON BIOMED RES INST,DEPT CELL BIOL,BOSTON,MA 02114
关键词
RECOMBINANT DNA; H+-TRANSLOCATING ATPASE; PHAGE-LAMBDA LIBRARY; SEQUENCING;
D O I
10.1016/0378-1119(91)90068-M
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Coupling factor 6 (F6) is a component of mitochondrial ATP synthase which is required for the interactions of the catalytic and proton-translocating segments. A human fetal muscle cDNA clone encoding this protein was isolated by screening a lambda-gt10 library with oligodeoxyribonucleotide probes. The 497-bp F6 cDNA included a 96-bp segment that delineated a presequence of 32 amino acids (aa) in the precursor protein, and 140 bp of 3'-untranslated sequence. The remainder of the cDNA sequence coded for a mature human F6 protein of 76 aa. The deduced primary aa sequence showed 81% homology to that of bovine F6, differing in 14 aa. Almost all of these aa substitutions were conservative and comparison of the hydropathy profiles revealed a similar pattern.
引用
收藏
页码:307 / 310
页数:4
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