PLANT-REGENERATION FROM PROTOPLASTS ISOLATED FROM COTYLEDONS OF SESBANIA-BISPINOSA

被引：0
|
作者：
ZHAO, YX
HARRIS, PJC
YAO, DY
机构：
[1] COVENTRY UNIV,DIV BIOL SCI,COVENTRY CV1 5FB,W MIDLANDS,ENGLAND
[2] SHANDONG TEACHERS UNIV,DEPT BIOL,JINAN,PEOPLES R CHINA
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 1995年 / 40卷 / 02期
关键词：
PROTOPLAST; REGENERATION; SESBANIA BISPINOSA;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Protoplasts were isolated from cotyledons of Sesbania bispinosa (Jacq.) W.F. Wight. In a liquid-over-agar culture system with Murashige and Skoog (MS) medium supplemented with 1 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D, 2 mg l(-1) benzyladenine (BA), 1 mg l(-1) glutamine and 0.5 M mannitol, 84% of these protoplasts divided and formed callus. The first division occurred after 3-4 days. Callus formed from the protoplasts differentiated shoots by organogenesis on MS medium with 1 mg l(-1) indolebutyric acid (IBA) and 1 mg l(-1) BA. These shoots developed into complete plantlets when excised and cultured on MS medium with 0.5 mg l(-1) IBA.
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页码：119 / 123
页数：5
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