MULTILOCUS ENZYME ELECTROPHORESIS USING ULTRATHIN POLYACRYLAMIDE GELS

被引：7
|
作者：
JOHN, MA [1 ]
HUSSAIN, Z [1 ]
机构：
[1] VICTORIA HOSP,DEPT CLIN MICROBIOL,LONDON N6A 4G5,ON,CANADA
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 1994年 / 19卷 / 04期
关键词：
BACTERIAL TYPING; MULTILOCUS ENZYME ELECTROPHORESIS; ULTRATHIN POLYACRYLAMIDE GEL;
D O I：
10.1016/0167-7012(94)90033-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Multilocus enzyme electrophoresis (MEE) using starch gels has been widely used to study bacterial genetics and for epidemiological typing. We describe a technique using ultrathin polyacrylamide gel electrophoresis (PAGE) which gives improved resolution and reproducibility, decreasing the number of gels which have to be run. We also describe a technique whereby several enzymes may be developed from a single running gel, resulting in significant time savings.
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页码：307 / 313
页数：7
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