RESPONSE-SURFACE OPTIMIZATION OF TEMPERATURE AND PH FOR THE GROWTH OF PACHYSOLEN-TANNOPHILUS

被引：17
|
作者：
ROEBUCK, K
BRUNDIN, A
JOHNS, M
机构：
[1] NORSK BIOFERM AS,SANDNES,NORWAY
[2] UNIV QUEENSLAND,DEPT CHEM ENGN,ST LUCIA,QLD 4067,AUSTRALIA
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 1995年 / 17卷 / 01期
关键词：
OPTIMIZATION; CELL PRODUCTION; PACHYSOLEN TANNOPHILUS;
D O I：
10.1016/0141-0229(94)00046-T
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Response surface modeling was applied to shake-flask cultures of Pachysolen tannophilus, CBS 4044, to determine the optimum temperature and pH for growth. A two-factorial experiment was designed and the results were analyzed and plotted using MODDE software. This technique proved efficient at identifying the optimum temperature and pH by performing a limited number of fermentations while varying the two variables at the same time. The results indicated that the maximum yield of biomass density corresponded to pH 3.7 and a temperature of 31.5 degrees C, at an initial carbon source (D-xylose) concentration of approximately 50 g l(-1).
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页码：75 / 78
页数：4
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