AMPEROMETRIC GLUCOSE BIOSENSOR BASED ON AN ELECTROCATALYTICALLY BULK-MODIFIED EPOXY-GRAPHITE BIOCOMPOSITE

被引：39
|
作者：
CESPEDES, F [1 ]
MARTINEZFABREGAS, E [1 ]
ALEGRET, S [1 ]
机构：
[1] UNIV AUTONOMA BARCELONA,DEPT QUIM,SENSORS & BIOSENSORS GRP,E-08193 BARCELONA,SPAIN
来源：
ANALYTICA CHIMICA ACTA | 1993年 / 284卷 / 01期
关键词：
AMPEROMETRY; BIOSENSORS; CATALYTIC METHODS; BIOCOMPOSITES; GLUCOSE BIOSENSOR; EPOXY-GRAPHITE BIOCOMPOSITE;
D O I：
10.1016/0003-2670(93)80003-4
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An inexpensive, robust, polishable and easily machinable amperometric glucose transducer was constructed. The biocomposite materials used were graphite, palladium-gold, non-conducting epoxy resin and glucose oxidase. The enzyme retains its bioactivity in the rigid epoxy-graphite matrix. The electrocatalytic oxidation of hydrogen peroxide is enhanced by gold-palladium and it is used as the analytical signal (at 900 mV vs. Ag/AgCl in a pH 7.00 buffered solution with 0.1 M phosphate and 0.1 M KCI). The biosensor exhibits a linear response to glucose in the 0.01-2 mM range and produces steady-state signals within a few seconds (6 s for 95% signal). Simple polishing procedures can be used to generate a fresh bioactive transducer surface.
引用
收藏
页码：21 / 26
页数：6
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