PRODUCTION OF SINGLE-STRANDED-DNA FOR SEQUENCING - AN ALTERNATIVE APPROACH

被引：0

作者：

MEDORI, R ^{[1
]}

TRITSCHLER, HJ ^{[1
]}

GAMBETTI, P ^{[1
]}

机构：

[1] CASE WESTERN RESERVE UNIV,INST PATHOL,DIV NEUROPATHOL,2085 ADELBERT RD,CLEVELAND,OH 44106

来源：

BIOTECHNIQUES | 1992年 / 12卷 / 03期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a simple procedure for the direct sequencing of single-stranded, PCR-amplified, target regions of human genomic DNA. At variance with previously reported procedures, purification of the desired double-stranded DNA was introduced. This additional step allowed the single-stranded amplification. and sequencing of the target gene. This step is required for direct sequencing of some amplified regions of human genomic DNA. However, no individual technique seems suitable to generate and sequence all single-stranded DNA.

引用

页码：346 / &

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