A SIMPLE METHOD FOR HEMOGLOBIN MEASUREMENT IN CELL-CULTURE SYSTEM

被引：0
|
作者：
BALIGA, BS [1 ]
ISOYAMA, K [1 ]
LONGMORE, G [1 ]
YANG, YM [1 ]
SHAH, AK [1 ]
MANKAD, VN [1 ]
机构：
[1] MIT,CAMBRIDGE,MA 02139
来源：
BLOOD CELLS | 1992年 / 18卷 / 02期
关键词：
HEMOGLOBIN ESTIMATION; K-562; MOUSE ERYTHROLEUKEMIC CELL LINE; BURST-FORMING UNIT ERYTHROID; HEMOGLOBIN ELECTROPHORESIS;
D O I：
暂无
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
A simple method of hemoglobin analysis in a cell culture system is described. Hemoglobins synthesized in cell cultures are labeled with radioactive amino acids. The cell extract containing radiolabeled hemoglobin is mixed with A, F, S, C, hemoglobin markers and separated by cellulose acetate electrophoresis. Individual bands of hemoglobin are cut from the gel and analyzed for radioactivity. This method is especially useful for determination of newly synthesized minute amount of hemoglobin in cell extracts that are difficult to visualize by staining procedure.
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页码：187 / 193
页数：7
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