CLONING AND SEQUENCE-ANALYSIS OF THE FLAGELLIN GENE OF CAMPYLOBACTER-JEJUNI TGH9011

被引：21

作者：

KHAWAJA, R ^{[1
]}

NEOTE, K ^{[1
]}

BINGHAM, HL ^{[1
]}

PENNER, JL ^{[1
]}

CHAN, VL ^{[1
]}

机构：

[1] UNIV TORONTO,DEPT MICROBIOL,FITZGERALD BLDG,150 COLL ST,TORONTO M5S 1A8,ONTARIO,CANADA

来源：

CURRENT MICROBIOLOGY | 1992年 / 24卷 / 04期

关键词：

D O I：

10.1007/BF01579284

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Flagella are essential for motility and have been implicated to be one of the pathogenic determinants. The flagellum of Campylobacter jejuni is a polymeric structure of a 62-kd protein. Using a high-affinity flagellin antibody to screen a lambda gt11 phage genomic expression library of C. jejuni strain TGH9011 (Serotype LIO36), a recombinant phage clone lambda gt11RK that expresses the C. jejuni flagellin protein was isolated. The recombinant lambda gt11RK produced a 56-kd protein upon induction with isopropylthiogalactoside, which reacted specifically with anti-flagellin antibody. The flagellin gene was sequenced, and comparative analysis of the nucleotide and amino acid sequence identified a region of the flagellin that shows hypervariability among different Campylobacter species and strains.

引用

页码：213 / 221

页数：9

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