MOLECULAR-CLONING OF RICE LACTATE-DEHYDROGENASE CDNA

A full length cDNA clone which encodes rice lactate dehydrogenase (LDH) was isolated from lambda gt11 library and sequenced. The LDH cDNA sequence shows 1026bp of the protein coding region, the 110bp of 5' flanking region and 240bp of 3' flanking region. The primary structure of LDH was deduced from nucleotide sequence. The LDH consists 353 amino acid residues and its molecular mass is deduced to be 38168. In the coding region of cDNA sequence, the rice LDH showed 75% homology with the barley LDHA and 54% with the human LDHB. The alignment of the whole amino acid sequence showed 85% and 45% homologies respectively with hose of the barley LDHA and the human LDHB.