CONCENTRATING HUMAN SPERM BEFORE CRYOPRESERVATION

被引：0
|
作者：
KOBAYASHI, T [1 ]
KANEKO, S [1 ]
HARA, I [1 ]
PARK, YJ [1 ]
SATO, H [1 ]
OHNO, T [1 ]
NOZAWA, S [1 ]
机构：
[1] TOKYO DENT COLL,ICHIKAWA GEN HOSP,DEPT OBSTET & GYNECOL,ICHIKAWA,CHIBA,JAPAN
来源：
ANDROLOGIA | 1991年 / 23卷 / 01期
关键词：
SPERMATOZOA; CRYOPRESERVATION; HUMAN;
D O I：
暂无
中图分类号：
R69 [泌尿科学（泌尿生殖系疾病）];
学科分类号：
摘要：
To improve the quality of cryo-preserved sperm, ejaculated semen was concentrated prior to freezing by means of continuous-step density gradient centrifugation. Freezing was simplified by employing liquid nitrogen vapor with KS-II cryo-medium. The original semen (n = 32, 41 +/- 13 x 10(6)/ml, 17 +/- 11% motility) was improved by the processing to be 68 +/- 29 x 10(6)/ml, 55 +/- 19% motility. Even after the specimens were thawed, sperm concentrations were similar to those of the original semen and 39 +/- 18% motility remained with a mean survival rate of 74 +/- 7.7%. The improved sperm motility and higher survival rate resulted in better post-thaw sperm quality than those of the original semen.
引用
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页码：25 / 28
页数：4
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