CONCENTRATING HUMAN SPERM BEFORE CRYOPRESERVATION

被引：0

作者：

KOBAYASHI, T ^{[1
]}

KANEKO, S ^{[1
]}

HARA, I ^{[1
]}

PARK, YJ ^{[1
]}

SATO, H ^{[1
]}

OHNO, T ^{[1
]}

NOZAWA, S ^{[1
]}

机构：

[1] TOKYO DENT COLL,ICHIKAWA GEN HOSP,DEPT OBSTET & GYNECOL,ICHIKAWA,CHIBA,JAPAN

来源：

ANDROLOGIA | 1991年 / 23卷 / 01期

关键词：

SPERMATOZOA; CRYOPRESERVATION; HUMAN;

D O I：

暂无

中图分类号：

R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

摘要：

To improve the quality of cryo-preserved sperm, ejaculated semen was concentrated prior to freezing by means of continuous-step density gradient centrifugation. Freezing was simplified by employing liquid nitrogen vapor with KS-II cryo-medium. The original semen (n = 32, 41 +/- 13 x 10(6)/ml, 17 +/- 11% motility) was improved by the processing to be 68 +/- 29 x 10(6)/ml, 55 +/- 19% motility. Even after the specimens were thawed, sperm concentrations were similar to those of the original semen and 39 +/- 18% motility remained with a mean survival rate of 74 +/- 7.7%. The improved sperm motility and higher survival rate resulted in better post-thaw sperm quality than those of the original semen.

引用

页码：25 / 28

页数：4

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