METHODS FOR DETERMINATION OF CELL-VOLUME IN TISSUE-CULTURE

被引：25
|
作者：
KIMELBERG, HK
OCONNOR, ER
SANKAR, P
KEESE, C
机构：
[1] UNION UNIV, DEPT PHARMACOL & TOXICOL, ALBANY, NY 12208 USA
[2] RENSSELAER POLYTECH INST, TROY, NY 12180 USA
来源：
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY | 1992年 / 70卷
关键词：
CELL VOLUME; MONOLAYER CULTURES; EXTRACELLULAR IMPEDANCE; RADIOLABELED INTRACELLULAR MARKERS; PRIMARY ASTROCYTE CULTURES;
D O I：
10.1139/y92-279
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
In this paper we present an overview of methods for determining cell volume in both suspension and monolayer cultures. Data from the use of selected methods such as the Coulter counter system for suspension cultures and radiolabelled intracellular markers for substratum-attached, monolayer cultures are presented. The advantages, limitations, and conditions under which the different methods can be used are discussed. It is pointed out that there is a need for more direct physical methods for measuring dynamic changes in the cell volume of monolayer cultures without removing the cells from the substratum. Data from a method applicable to such cultures that measures extracellular impedance are presented.
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页码：S323 / S333
页数：11
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