SYNTHESIS OF C-11 LABELED SCH-39166, A NEW SELECTIVE DOPAMINE D-1 RECEPTOR LIGAND, AND PRELIMINARY PET INVESTIGATIONS

SCH 39166 [(-)-trans-6,7,7a,8,9,13b-hexahydro-3-chloro-2-hydroxy-N-methyl-5H-benzo(d)naphtho-(2,1-b)azepine] is a new more selective dopamine D-1 receptor antagonist than the widely used SCH 23390. [C-11]SCH 39166 was prepared by N-methylation of the desmethyl compound SCH 40853 [(-)-trans-6,7,7a,8,9,13b-hexahydro-3-chloro-2-2-hydroxy-5H-benzo(d)naphtho-(2,1-b)azepine] with [C-11]methyl iodine. Reaction in acetone with subsequent straight-phase semi-preparative HPLC resulted in 20-30% radiochemical yield (from EOB and decay-corrected) with a total synthesis time of 35-40 min and a radiochemical purity > 99%. The specific activity obtained at EOS was about 1500 Ci/mmol (55 GBq/mu-mol). [C-11]SCH 39166 was injected into a Cynomolgus monkey. PET-analysis demonstrated accumulation in the striatum, a region known to have a high density of dopamine D-1 receptors. In a displacement experiment, radioactivity in the straitum was markedly reduced after injection of 6 mg unlabelled SCH 23390, thus demonstrating the specificity and reversibility of [C-11]SCH 39166 binding to dopamine D-1 receptors.