NEW ASSAY TO DETECT SHEDDING ANTIGEN WITH USE OF ANTIIDIOTYPIC MONOCLONAL-ANTIBODIES

被引：1

作者：

TSUJISAKI, M ^{[1
]}

IMAI, K ^{[1
]}

TOKUCHI, S ^{[1
]}

MASUYA, J ^{[1
]}

ISHIDA, T ^{[1
]}

ADACHI, M ^{[1
]}

HINODA, Y ^{[1
]}

HANZAWA, Y ^{[1
]}

YACHI, A ^{[1
]}

机构：

[1] AJINOMOTO CO INC,LIFE SCI LAB,KAWASAKI,KANAGAWA 210,JAPAN

来源：

JOURNAL OF CLINICAL LABORATORY ANALYSIS | 1991年 / 5卷 / 01期

关键词：

ANTIIDIOTYPIC MONOCLONAL ANTIBODY; CARCINOEMBRYONIC ANTIGEN; SHEDDING ANTIGEN; IDIOTYPE MAPPING; IDIOTYPE-ANTI-IDIOTYPE INTERACTION;

D O I：

10.1002/jcla.1860050104

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Anti-idiotypic (Anti-Id) monoclonal antibodies (MoAbs) against anti-carcinoembryonic antigen (CEA) monoclonal antibody 5B3 (IgG1) were prepared and characterized. Five anti-Id MoAbs recognized the private idiotype of MoAb 5B3. Idiotype mapping suggested that MoAb 5B3 had at least three distinct idiotopes at its antigen combining site. Utilizing the inhibition of idiotype-anti-idiotype interaction with use of anti-Id MoAb T4-305 or T4-212, an immunoassay to detect shedding CEA was established. The results of this inhibition assay correlated with those of double determinant immunoassay and inhibition percentages linearly increased in a CEA concentration-dependent manner. Since it requires only one epitope of a given antigen molecule, this assay could be widely used for detection of shedding antigens, including tumor-associated antigens.

引用

页码：14 / 20

页数：7

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