INVESTIGATION OF THE REPAIR OF SINGLE-STRAND BREAKS IN HUMAN DNA USING ALKALINE GEL-ELECTROPHORESIS

被引：6
|
作者：
KOVACS, E
LANGEMANN, H
机构：
来源：
RADIATION RESEARCH | 1990年 / 124卷 / 02期
关键词：
D O I：
10.2307/3577857
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Unstimulated lymphocytes from eight healthy persons were exposed to 10-, 30-, and 100-Gy doses of 60Co γ radiation. The repair of damaged DNA was measured by (1) alkaline gel electrophoresis (extracted DNA loaded on 0.25% agarose gel, run at 1 V/cm for 39-44 h) at 0, 1, and 2 h after exposure and (2) incorporation of [3H]thymidine into unstimulated lymphocytes in the presence of 2 mM hydroxyurea 1 and 2 h after exposure. Both methods - alkaline gel electrophoresis and thymidine incorporation - showed that repair was completed within 2 h.
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页码：137 / 140
页数：4
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