INVESTIGATION OF THE REPAIR OF SINGLE-STRAND BREAKS IN HUMAN DNA USING ALKALINE GEL-ELECTROPHORESIS

被引：6
|
作者：
KOVACS, E
LANGEMANN, H
机构：
来源：
RADIATION RESEARCH | 1990年 / 124卷 / 02期
关键词：
D O I：
10.2307/3577857
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Unstimulated lymphocytes from eight healthy persons were exposed to 10-, 30-, and 100-Gy doses of 60Co γ radiation. The repair of damaged DNA was measured by (1) alkaline gel electrophoresis (extracted DNA loaded on 0.25% agarose gel, run at 1 V/cm for 39-44 h) at 0, 1, and 2 h after exposure and (2) incorporation of [3H]thymidine into unstimulated lymphocytes in the presence of 2 mM hydroxyurea 1 and 2 h after exposure. Both methods - alkaline gel electrophoresis and thymidine incorporation - showed that repair was completed within 2 h.
引用
收藏
页码：137 / 140
页数：4
相关论文
共 50 条
  • [1] DETECTION OF POLYMORPHISMS OF HUMAN DNA BY GEL-ELECTROPHORESIS AS SINGLE-STRAND CONFORMATION POLYMORPHISMS
    ORITA, M
    IWAHANA, H
    KANAZAWA, H
    HAYASHI, K
    SEKIYA, T
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (08) : 2766 - 2770
  • [2] SINGLE-STRAND BREAKS IN SUPERCOILED DNA BY VACUUM AND FAR ULTRA-VIOLET LIGHT BY GEL-ELECTROPHORESIS
    TAKAKURA, K
    ISHIKAWA, M
    JOURNAL OF RADIATION RESEARCH, 1983, 24 (01) : 66 - 66
  • [3] Estimation of DNA single-strand breaks by single cells gel electrophoresis in tumor cells
    Neubauer, S
    Liehr, T
    Birkenhake, S
    Gebhart, E
    Fietkau, R
    Sauer, R
    GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING, 1998, 14 (04): : 121 - 124
  • [4] ENZYMATIC REPAIR OF SINGLE-STRAND BREAKS IN DNA
    WEISS, B
    LIVE, TR
    RICHARDS.CC
    FEDERATION PROCEEDINGS, 1967, 26 (02) : 395 - &
  • [5] REPRODUCIBILITY OF BASAL AND INDUCED DNA SINGLE-STRAND BREAKS DETECTED BY THE SINGLE-CELL GEL-ELECTROPHORESIS ASSAY IN HUMAN PERIPHERAL MONONUCLEAR LEUKOCYTES
    HOLZ, O
    JORRES, A
    KASTNER, A
    KRAUSE, T
    MAGNUSSEN, H
    INTERNATIONAL ARCHIVES OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH, 1995, 67 (05) : 305 - 310
  • [6] Targeting single-strand DNA breaks for DNA repair
    Gerasimova, N.
    Maluchenko, N.
    Sultanov, D.
    Valieva, M.
    Kotova, E.
    Feofanov, A.
    Studitsky, V.
    FEBS OPEN BIO, 2018, 8 : 144 - 144
  • [7] GEL-ELECTROPHORESIS METHOD FOR QUANTITATION OF GAMMA-RAY INDUCED SINGLE-STRAND AND DOUBLE-STRAND BREAKS IN DNA IRRADIATED INVITRO
    CHEN, CZ
    SUTHERLAND, JC
    ELECTROPHORESIS, 1989, 10 (5-6) : 318 - 326
  • [8] REPAIR OF DNA SINGLE-STRAND BREAKS IN HUMAN CELLS WITH GENETIC DISORDERS
    TRETON, J
    BOUCAYS, A
    DERSAKISSIAN, H
    BOUE, A
    COURTOIS, Y
    CELL BIOLOGY INTERNATIONAL REPORTS, 1978, 2 (04) : 403 - 410
  • [9] REPAIR OF DNA SINGLE-STRAND BREAKS IN NEURONS: IMPLICATIONS ON HUMAN HEALTH
    El-Khamisy, Sherif
    Caldecott, Keith
    CELLULAR ONCOLOGY, 2009, 31 (02) : 131 - 131
  • [10] Evaluation of three methods for the detection of DNA single-strand breaks in human lymphocytes: Alkaline elution, nick translation, and single-cell gel electrophoresis
    Leroy, T
    VanHummelen, P
    Anard, D
    Castelain, P
    KirschVolders, M
    Lauwerys, R
    Lison, D
    JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH, 1996, 47 (05): : 409 - 422
12345