SIMPLE AND RAPID 2-STEP POLYMERASE CHAIN-REACTION FOR DIAGNOSIS OF PNEUMOCYSTIS-CARINII INFECTION

被引：26
|
作者：
TAMBURRINI, E [1 ]
MENCARINI, P [1 ]
DELUCA, A [1 ]
ANTINORI, A [1 ]
VISCONTI, E [1 ]
AMMASSARI, A [1 ]
ORTONA, L [1 ]
ORTONA, E [1 ]
SIRACUSANO, A [1 ]
VICARI, G [1 ]
机构：
[1] IST SUPER SANITA, DEPT IMMUNOL, I-00161 ROME, ITALY
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1993年 / 31卷 / 10期
关键词：
D O I：
10.1128/JCM.31.10.2788-2789.1993
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Two amplification steps were made to detect Pneumocystis carinii DNA by polymerase chain reaction (PCR). pAZ102-E and pAZ102-H (standard PCR), pAZ102-L2 (sense), and pAZ102-E (antisense) (two-step PCR) were used as primers. The amplification products were analyzed by ethidium bromide. After the two-step PCR, ethidium bromide detected all samples positive by oligohybridization after one amplification step. Our two-step PCR is a rapid, cost-effective, and clinically suitable method for the detection of P. carinii infection.
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页码：2788 / 2789
页数：2
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