SIMPLE AND RAPID 2-STEP POLYMERASE CHAIN-REACTION FOR DIAGNOSIS OF PNEUMOCYSTIS-CARINII INFECTION

被引：26

作者：

TAMBURRINI, E ^{[1
]}

MENCARINI, P ^{[1
]}

DELUCA, A ^{[1
]}

ANTINORI, A ^{[1
]}

VISCONTI, E ^{[1
]}

AMMASSARI, A ^{[1
]}

ORTONA, L ^{[1
]}

ORTONA, E ^{[1
]}

SIRACUSANO, A ^{[1
]}

VICARI, G ^{[1
]}

机构：

[1] IST SUPER SANITA, DEPT IMMUNOL, I-00161 ROME, ITALY

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1993年 / 31卷 / 10期

关键词：

D O I：

10.1128/JCM.31.10.2788-2789.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two amplification steps were made to detect Pneumocystis carinii DNA by polymerase chain reaction (PCR). pAZ102-E and pAZ102-H (standard PCR), pAZ102-L2 (sense), and pAZ102-E (antisense) (two-step PCR) were used as primers. The amplification products were analyzed by ethidium bromide. After the two-step PCR, ethidium bromide detected all samples positive by oligohybridization after one amplification step. Our two-step PCR is a rapid, cost-effective, and clinically suitable method for the detection of P. carinii infection.

引用

页码：2788 / 2789

页数：2

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