Proliferation and differentiation of human fetal brain neural stem cells in vitro

被引：10
|
作者：
McGrath, Erica L. [1 ]
Gao, Junling [1 ]
Wu, Ping [1 ,2 ]
机构：
[1] Univ Texas Med Branch, Dept Neurosci & Cell Biol, Res Bldg 17,Room 4-212 B,301 Univ Blvd, Galveston, TX 77555 USA
[2] Capital Med Univ, Beijing Inst Brain Disorders, Beijing, Peoples R China
来源：
JOURNAL OF NEURORESTORATOLOGY | 2018年 / 6卷
关键词：
neural stem cell; proliferation; differentiation; neuron; astrocyte;
D O I：
10.2147/JN.S148794
中图分类号：
R74 [神经病学与精神病学];
学科分类号：
摘要：
Primary human fetal brain neural stem cells (hNSCs) are a unique non-genetically modified model system to study molecular mechanisms underlying human neural development, to model human diseases, and to screen drugs or validate new treatments. They may also be used for cell transplantation to treat various neurological diseases. This protocol details our methods that can be used to expand hNSCs in culture as well as how to differentiate them into various neuronal lineages and astrocytes.
引用
收藏
页码：19 / 27
页数：9
相关论文
共 50 条
  • [21] Human amniotic fluid stem cells: neural differentiation in vitro and in vivo
    Tullia Maraldi
    Laura Bertoni
    Massimo Riccio
    Manuela Zavatti
    Gianluca Carnevale
    Elisa Resca
    Marianna Guida
    Francesca Beretti
    Giovanni B. La Sala
    Anto De Pol
    Cell and Tissue Research, 2014, 357 : 1 - 13
  • [22] Effect of VEGF on Neural Differentiation of Human Embryonic Stem Cells in vitro
    Jiao, Shujie
    Xu, Huifang
    Xu, Jie
    Zhan, Yanqiang
    Zhang, Suming
    JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES, 2009, 29 (05) : 563 - 566
  • [23] Effect of VEGF on neural differentiation of human embryonic stem cells in vitro
    Shujie Jiao
    Huifang Xu
    Jie Xu
    Yanqiang Zhan
    Suming Zhang
    Journal of Huazhong University of Science and Technology [Medical Sciences], 2009, 29 : 563 - 566
  • [24] Human amniotic fluid stem cells: neural differentiation in vitro and in vivo
    Maraldi, Tullia
    Bertoni, Laura
    Riccio, Massimo
    Zavatti, Manuela
    Carnevale, Gianluca
    Resca, Elisa
    Guida, Marianna
    Beretti, Francesca
    La Sala, Giovanni B.
    De Pol, Anto
    CELL AND TISSUE RESEARCH, 2014, 357 (01) : 1 - 13
  • [25] Fetal hepatocyte stimulate differentiation of human mesenchymal stem cells into hepatocytes in vitro
    Yuanmei Che
    Yang Wang
    Yi Zhang
    Cell Research, 2008, 18 : S145 - S145
  • [26] Fetal hepatocyte stimulate differentiation of human mesenchymal stem cells into hepatocytes in vitro
    Che, Yuanmei
    Wang, Yang
    Zhang, Yi
    CELL RESEARCH, 2008, 18 (Suppl 1) : S145 - S145
  • [27] Lowered glucose suppressed the proliferation and increased the differentiation of murine neural stem cells in vitro
    Horie, N
    Moriya, T
    Mitome, M
    Kitagawa, N
    Nagata, I
    Shinohara, K
    FEBS LETTERS, 2004, 571 (1-3): : 237 - 242
  • [28] Mechanism of leptin regulating proliferation and differentiation of rat embryonic neural stem cells in vitro
    Tan, Ruolan
    An, Jing
    Cai, Zhenlu
    Hu, Xiaoxuan
    Sun, Meiqi
    Ma, Ning
    Lu, Haixia
    JOURNAL OF ANATOMY, 2020, 236 : 195 - 196
  • [29] Effects of Gastrodiae rhizoma on proliferation and differentiation of human embryonic neural stem cells
    Baral, Samrat
    Pariyar, Ramesh
    Yoon, Chi-Su
    Kim, Dong-Cheol
    Yun, Jong-Min
    Jang, Seok O.
    Kim, Sung Yeon
    Oh, Hyuncheol
    Kim, Youn-Chul
    Seo, Jungwon
    ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE, 2015, 8 (10) : 776 - 781
  • [30] Effects of Gastrodiae rhizoma on proliferation and differentiation of human embryonic neural stem cells
    Samrat Baral
    Ramesh Pariyar
    Chi-Su Yoon
    Dong-Cheol Kim
    Jong-Min Yun
    Seok O Jang
    Sung Yeon Kim
    Hyuncheol Oh
    Youn-Chul Kim
    Jungwon Seo
    Asian Pacific Journal of Tropical Medicine, 2015, (10) : 776 - 781
12345