A RAPID METHOD FOR THE ANALYSIS OF INFLUENZA-VIRUS GENES - APPLICATION TO THE REASSORTMENT OF EQUINE INFLUENZA-VIRUS GENES

被引：33
|
作者：
ADEYEFA, CAO
QUAYLE, K
MCCAULEY, JW
机构：
[1] AFRC,INST ANIM HLTH,COMPTON LAB,NEWBURY RG16 0NN,BERKS,ENGLAND
[2] AFRC,INST ANIM HLTH,PIRBRIGHT LAB,WOKING GU24 0NF,SURREY,ENGLAND
来源：
VIRUS RESEARCH | 1994年 / 32卷 / 03期
关键词：
INFLUENZA VIRUS; EQUINE INFLUENZA; PCR; MULTIPLEX-PCR; GENE REASSORTMENT;
D O I：
10.1016/0168-1702(94)90087-6
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We describe a rapid method for genetic characterisation of influenza virus genes using reverse transcription and amplification by polymerase chain reaction (RT/PCR) of all virus segments simultaneously (multiplex RT/PCR) using primers based on the conserved terminal sequences. The product has been shown to be suitable for determination of partial nucleotide sequences which can be used to search nucleotide sequence databases and rapidly map the genetic origin of each segment. We illustrate the use of the method by analysing genetic reassortment in H7N7 equine influenza viruses.
引用
收藏
页码：391 / 399
页数：9
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