Cyclomaltodextrin glucotransferases from thermophilic actinomycetes

被引：0
|
作者：
Abelian, VA
Afyan, KB
Avakian, ZG
Melkumyan, AG
Afrikian, EG
机构：
来源：
BIOCHEMISTRY-MOSCOW | 1995年 / 60卷 / 10期
关键词：
cyclomaltodextrin glucotransferase; cyclodextrins; actinomycetes;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cyclomaltodextrin glucotransferases (CGTases, EC 2.4.1.19) were identified in strains of thermophilic actinomycetes for the first time. The enzymes were purified to homogeneity using adsorption on a mixed alpha-and beta-cyclodextrin (CD) polymer followed by ion-exchange and gel-permeation chromatography. Optimal conditions for the catalytic activity of the enzymes (pH 6-7, 50-60 degrees C) were determined. For the CGTases from strains INMIA-A-554 and INMIA-A-561, respectively, the K-m values were 5.0 and 3.33 mg/ml (with starch) and the V-max values were 10.0 and 6.67 mg/min. The substrate and acceptor specificities of the enzymes were studied. The presence of CGTases in thermophilic actinomycetes points to their phylogenetic relationship to aerobic spore-forming bacteria.
引用
收藏
页码：1223 / 1229
页数：7
相关论文
共 50 条
  • [41] SCREENING OF THERMOPHILIC ACTINOMYCETES FOR BIOPOLYMER DEGRADING ENZYMES
    KEMPF, A
    KUTZNER, HJ
    DECHEMA BIOTECHNOLOGY CONFERENCES, VOL 3, PTS A AND B, 1989, 3 : 159 - 162
  • [42] THERMOPHILIC FERMENTATION OF PIG FECES AND STRAW BY ACTINOMYCETES
    GINNIVAN, MJ
    WOODS, JL
    OCALLAGHAN, JR
    JOURNAL OF APPLIED BACTERIOLOGY, 1977, 43 (02): : 231 - 238
  • [43] Degradation of poly(tetramethylene succinate) by thermophilic actinomycetes
    Jarerat, A
    Tokiwa, Y
    BIOTECHNOLOGY LETTERS, 2001, 23 (08) : 647 - 651
  • [44] Isolation of thermophilic actinomycetes producers of thermostable proteases
    Hristova, I.
    Nedelcheva, P.
    Gushterova, A.
    Paskaleva, D.
    Krastanov, A.
    MICROBES IN APPLIED RESEARCH: CURRENT ADVANCES AND CHALLENGES, 2012, : 423 - 426
  • [45] PRESENCE OF THERMOPHILIC ACTINOMYCETES IN RESIDENTIAL HEATING SYSTEMS
    FINK, JN
    RESNICK, AJ
    SALVAGGIO, J
    APPLIED MICROBIOLOGY, 1971, 22 (04) : 730 - +
  • [46] Growth and morphological appearance of thermotolerant and thermophilic actinomycetes
    Albrecht, A
    Kämpfer, P
    GEFAHRSTOFFE REINHALTUNG DER LUFT, 2000, 60 (04): : 139 - 145
  • [47] ISOLATION PROPERTIES AND POTENTIAL APPLICATIONS OF THERMOPHILIC ACTINOMYCETES
    EDWARDS, C
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1993, 42 (2-3) : 161 - 179
  • [48] Degradation of poly(tetramethylene succinate) by thermophilic actinomycetes
    Amnat Jarerat
    Yutaka Tokiwa
    Biotechnology Letters, 2001, 23 : 647 - 651
  • [49] HYPERSENSITIVITY PNEUMONITIS DUE TO SENSITIZATION WITH THERMOPHILIC ACTINOMYCETES
    KURUP, VP
    IMMUNOLOGY AND ALLERGY CLINICS OF NORTH AMERICA, 1989, 9 (02) : 285 - 306
  • [50] OCCURRENCE OF THERMOPHILIC ACTINOMYCETES IN NATURAL SUBSTRATES IN NIGERIA
    UNAOGU, IC
    GUGNANI, HC
    LACEY, J
    ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 1994, 65 (01): : 1 - 5
12345