ROLE OF BRADYKININ IN THE REGULATION OF BLOOD-PRESSURE AND RENAL BLOOD-FLOW IN DOCA-SALT HYPERTENSIVE RATS

We examined the role of bradykinin in the onset and/or the maintenance of blood pressure and renal blood flow in deoxycorticosterone acetate (DOCA)-salt hypertensive rats by using a competitive antagonist of bradykinin [Arg—Pro—Hyp—Gly— Thi—Ser—Dphe—Thi—Arg; Hyp, L-4-hydroxyproline; Thi, β-(2-theinyl-L-alanine)L The intravenous injection of the bradykinin antagonist (25, 50 and 100 µg) produced an increase in mean arterial pressure in all rats treated with tap water, 1% NaCl and DOCA + 1% NaCI. However, the magnitude of the increase in mean arterial pressure was significantly lower in the DOCA-hypertensive rats than in the two groups of rats drinking tap water and 1% NaCl after 4 and 6 weeks, but there was no significant difference after 2 weeks. The bradykinin antagonist induced a decrease in renal blood flow in all rats. However, the extent of the fall in renal blood flow was reduced in the DOCA-hypertensive rats compared with the control rats drinking tap water. These results suggest that endogenous bradykinin is depressed in the established phase of hypertension in DOCA-hypertensive rats. It is also suggested that endogenous bradykinin may counteract the elevation of vascular resistance in the early stages of this model. © Current Science Ltd.