INVITRO DEFINITION OF THE YEAST RNA POLYMERASE-I PROMOTER

被引：37
|
作者：
CHOE, SY [1 ]
SCHULTZ, MC [1 ]
REEDER, RH [1 ]
机构：
[1] HUTCHINSON CANC RES CTR,1124 COLUMBIA ST,SEATTLE,WA 98104
来源：
NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 02期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1093/nar/20.2.279
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The structure of the ribosomal gene promoter from Saccharomyces cerevisiae has been analyzed in a whole cell in vitro extract. The promoter contains at least two essential domains, an upstream domain located at the 5' boundary near position -150 and a core promoter domain around the site of transcription initiation at +1. The upstream domain augments transcription in vitro but is not absolutely required. Maintenance of correct spacing between the two domains is critical. The in vitro analysis agrees well with prior in vivo analysis and it appears that the yeast promoter has a structure very similar to that of vertebrate ribosomal gene promoters.
引用
收藏
页码：279 / 285
页数：7
相关论文
共 50 条
12345