THE USE OF FED-BATCH CULTIVATION FOR ACHIEVING HIGH CELL DENSITIES IN THE PRODUCTION OF A RECOMBINANT PROTEIN IN ESCHERICHIA-COLI

被引：8

作者：

STRANDBERG, L ^{[1
]}

ANDERSSON, L ^{[1
]}

ENFORS, SO ^{[1
]}

机构：

[1] ROYAL INST TECHNOL,DEPT BIOCHEM & BIOTECHNOL,S-10044 STOCKHOLM,SWEDEN

来源：

FEMS MICROBIOLOGY REVIEWS | 1994年 / 14卷 / 01期

关键词：

ESCHERICHIA COLI; RECOMBINANT PROTEIN; FED BATCH; HIGH CELL DENSITY; MAINTENANCE ENERGY; VIABILITY;

D O I：

10.1016/0168-6445(94)90013-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The production of the fusion protein staphylococcal protein A/E. coli beta-galactosidase in Escherichia coli was studied in batch and fed batch cultivations. Batch cultivation of a recombinant E. coli strain yielded a final cell dry weight of 16.4 g l(-1) with a final intracellular product concentration of recombinant protein corresponding to approximately 38% of the cell dry weight. Fed batch cultivation made it possible to increase the final cell dry weight to 77.0 g l(-1). The intracellular product concentration (25%) was lower as compared to batch cultivation resulting in a total concentration of recombinant protein of 19.2 g l(-1).

引用

页码：53 / 56

页数：4

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