The renaturation kinetics of nuclear DNA from the G3 colony of Anopheles gambiae Giles was studied to estimate the genome size and to determine the proportion of repeated sequences. An. gambiae has a haploid DNA content of 0.27 picograms or 2.6 x 10(8) basepairs. Analysis of reassociation kinetics indicated that the genome is composed of 61% single-copy and 33% repetitive sequences, with 6% foldback sequences.
机构:Indiana Univ, Dept Biol, Howard Hughes Med Inst, Bloomington, IN 47405 USA
Powers, TP
Hogan, J
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机构:Indiana Univ, Dept Biol, Howard Hughes Med Inst, Bloomington, IN 47405 USA
Hogan, J
Ke, ZX
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机构:Indiana Univ, Dept Biol, Howard Hughes Med Inst, Bloomington, IN 47405 USA
Ke, ZX
Dymbrowski, K
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机构:Indiana Univ, Dept Biol, Howard Hughes Med Inst, Bloomington, IN 47405 USA
Dymbrowski, K
Wang, XL
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机构:Indiana Univ, Dept Biol, Howard Hughes Med Inst, Bloomington, IN 47405 USA
Wang, XL
Collins, FH
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机构:Indiana Univ, Dept Biol, Howard Hughes Med Inst, Bloomington, IN 47405 USA
Collins, FH
Kaufman, TC
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Indiana Univ, Dept Biol, Howard Hughes Med Inst, Bloomington, IN 47405 USAIndiana Univ, Dept Biol, Howard Hughes Med Inst, Bloomington, IN 47405 USA