REASSOCIATION KINETICS OF ANOPHELES-GAMBIAE (DIPTERA, CULICIDAE) DNA

被引:47
|
作者
BESANSKY, NJ [1 ]
POWELL, JR [1 ]
机构
[1] YALE UNIV, DEPT BIOL, NEW HAVEN, CT 06511 USA
关键词
INSECTA; ANOPHELES-GAMBIAE; GENOME SIZE; REASSOCIATION KINETICS;
D O I
10.1093/jmedent/29.1.125
中图分类号
Q96 [昆虫学];
学科分类号
摘要
The renaturation kinetics of nuclear DNA from the G3 colony of Anopheles gambiae Giles was studied to estimate the genome size and to determine the proportion of repeated sequences. An. gambiae has a haploid DNA content of 0.27 picograms or 2.6 x 10(8) basepairs. Analysis of reassociation kinetics indicated that the genome is composed of 61% single-copy and 33% repetitive sequences, with 6% foldback sequences.
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页码:125 / 128
页数:4
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