DETERMINATION OF N-ACETYLGLUCOSAMINYLTRANSFERASE-III, N-ACETYLGLUCOSAMINYLTRANSFERASE-IV AND N-ACETYLGLUCOSAMINYLTRANSFERASE-V IN NORMAL AND HEPATOMA TISSUES OF RATS

被引：109
|
作者：
NISHIKAWA, A [1 ]
GU, J [1 ]
FUJII, S [1 ]
TANIGUCHI, N [1 ]
机构：
[1] OSAKA UNIV,SCH MED,DEPT BIOCHEM,4-3-57 NAKANOSHIMA,KITA KU,TOYONAKA,OSAKA 560,JAPAN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1035卷 / 03期
关键词：
(Rat); 2-Aminopyridine; HPLC; N-Acetylglucosaminyltransferase;
D O I：
10.1016/0304-4165(90)90094-D
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
N-Acetylglucosaminyltransferase III, IV and V activities were assayed in various rat tissues and hepatomas using the same fluorescence-labeled sugar chain, GlcNAcβ1-2Manα1-3(GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAc-2-aminopyridine as a substrate. The N-acetylglucosaminyltransferase II activity toward the substrate is the highest in most rat tissues including primary rat hepatoma. A relatively higher activity for GnT-V is found in small intestine, serum and hepatoma as compared to that of GnT-IV. Some kinetic properties of these enzymes in crude extracts were also determined. © 1990.
引用
收藏
页码：313 / 318
页数：6
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