METHOD FOR THE PREPARATION OF HEAVY-ATOM DERIVATIVES OF YEAST CYTOCHROME-C PEROXIDASE

被引：2
|
作者：
ERIKSSON, B
ERSSON, B
KIERKEGAARD, P
LARSSON, LO
SKOGLUND, U
LUDWIG, M
YONETANI, T
机构：
[1] UNIV MICHIGAN, DIV BIOPHYS RES, ANN ARBOR, MI 48104 USA
[2] UNIV PENN, SCH MED, DEPT BIOCHEM & BIOPHYS, PHILADELPHIA, PA 19174 USA
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1979年 / 127卷 / 02期
关键词：
D O I：
10.1016/0022-2836(79)90243-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A method for the preparation of heavy-atom derivatives is described. It has succeeded with yeast cytochrome c peroxidase and may be useful for other crystalline proteins. Transfer of the crystals from methylpentanediol solutions (in which they were grown) to solutions of polyethylene glycol (MW 6000) maintains the crystal structure so that crystals tolerate heavy-atom concentrations at least 10 times larger than could be attained in methylpentanediol mother liquors.
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页码：225 / 227
页数：3
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