Selective blood-DNA extraction from mixed stain using ABO antibody for short tandem repeat typing

被引：3
|
作者：
Honda, Katsuya [1 ]
Yano, Shizue [1 ]
Nishi, Takeki [1 ]
Iwabuchi, Yayoi [1 ]
Kurosu, Akira [1 ]
Sugano, Yukiko [1 ]
机构：
[1] Univ Tsukuba, Fac Med, Dept Legal Med, Tsukuba, Ibaraki, Japan
来源：
FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES | 2013年 / 4卷 / 01期
关键词：
Antibody; Bloodstain; DNA extraction; ABO;
D O I：
10.1016/j.fsigss.2013.10.166
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Short tandem repeat (STR) typing of DNA extracted from various kinds of mixed samples can be difficult because the origin of the blood donor cannot be easily discriminated. We present a method for the extraction of DNA from mixed bloodstains involving plural contributors, after antibody captured cell separation. This method has proven highly successful in the recovery of DNA profiles corresponding to ABO blood type. The methodological steps presented here are simple: leukocyte agglutination by ABO antibody followed by centrifugal separation of agglutinated leukocytes. Using this method, selective DNA can be extracted from mixtures of blood and/or different cells, using cell specific antibodies. The method presented here is applicable to DNA-based identification of various kinds of contaminated samples. (C) 2013 Elsevier Ireland Ltd. All rights
引用
收藏
页码：E326 / E327
页数：2
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