ANALYSIS OF HYDROXYPROLINE IN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AFTER DABSYL-CHLORIDE DERIVATIZATION

被引：3
|
作者：
IKEDA, M
SORIMACHI, K
AKIMOTO, K
OKAZAKI, M
SUNAGAWA, M
NIWA, A
机构：
[1] DOKKYO UNIV,SCH MED,DEPT MICROBIOL,MIBU,TOCHIGI 32102,JAPAN
[2] DOKKYO UNIV,SCH MED,CELLULAR & MOLEC BIOL RES CTR,MIBU,TOCHIGI 32102,JAPAN
[3] DOKKYO UNIV,SCH MED,DEPT SURG,MIBU,TOCHIGI 32102,JAPAN
来源：
AMINO ACIDS | 1995年 / 8卷 / 04期
关键词：
AMINO ACIDS; HYDROXYPROLINE; DABSYL-CHLORIDE; URINE; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; O-PHTHALALDEHYDE;
D O I：
10.1007/BF00806559
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In order to analyse hydroxyproline (HYP) in urine, a high-performance liquid chromatographic method was modified. The primary amino groups were blocked with o-phthalaldehyde, and then the secondary amino groups were derivatized with 4-dimethylaminoazobenzene-4'-sulphonyl chloride. In addition, the dabsylated samples were treated with ethyl acetate to obtain a simple elution profile in high-performance liquid chromatography. The dabsyl-HYP and -proline were eluted at 4.7 min and 8.0 min, respectively. The chromatographic analysis was completed within 10 min, including the time needed for reequilibration of the column. Using the present method, the concentration of HYP in urine was determined to 260 +/- 6 mu mol/l.
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页码：401 / 407
页数：7
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