ISOLATION AND PARTIAL CHARACTERIZATION OF SUBPOPULATIONS OF ALVEOLAR MACROPHAGES, GRANULOCYTES, AND HIGHLY ENRICHED INTERSTITIAL MACROPHAGES FROM RAT LUNG

被引:73
|
作者
LAVNIKOVA, N [1 ]
PROKHOROVA, S [1 ]
HELYAR, L [1 ]
LASKIN, DL [1 ]
机构
[1] RUTGERS STATE UNIV,DEPT PHARMACOL & TOXICOL,POB 789,PISCATAWAY,NJ 08855
关键词
D O I
10.1165/ajrcmb/8.4.384
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The contribution of interstitial macrophages (IM) to lung defense, homeostasis, and pathophysiology is for the most part unknown. Studies on this cell type are difficult because they are not readily accessible in large numbers or in high purity. In the present work, various nonenzymatic and enzymatic methods were compared with the aim of isolating and characterizing pure populations of lung IM. The results of our studies demonstrate that most procedures currently used to isolate IM yield subpopulations of alveolar macrophages (AM) or IM highly contaminated by AM and granulocytes. We found that lavage of the lung yielded only one half of the total AM present in the tissue. The remainder of the AM could only be obtained by extensive washing of cut and disaggregated lung tissue, which is considered by some investigators to be an effective procedure for IM isolation. According to our results, cells recovered by lavage and washing of cut and disaggregated lung tissue were morphologically and histochemically identical, were strongly positive for nonspecific esterase, highly phagocytic, and appeared to represent subpopulations of AM with apparently varying degrees of adherence to the alveolar walls. We also found that IM could be obtained in high purity by sequential digestion of the remaining lung tissue with 60 and 175 IU/ml of collagenase followed by selective adherence. Digestion of the tissue with 60 IU/ml of collagenase resulted in a highly enriched population of granulocytes and also reduced their contamination in the IM population. The resulting IM were distinct from AM by morphology and histochemistry. Like AM, these cells displayed Fc receptor-mediated phagocytosis. The results of the present studies provide a unique and significantly shorter method for isolating highly enriched populations of IM and granulocytes from rat lung without time-consuming and inefficient lung lavage procedures.
引用
收藏
页码:384 / 392
页数:9
相关论文
共 50 条
  • [21] Extensive surface phenotyping of alveolar macrophages in interstitial lung disease
    Taylor, ML
    Noble, PW
    White, B
    Wise, R
    Liu, MC
    Bochner, BS
    CLINICAL IMMUNOLOGY, 2000, 94 (01) : 33 - 41
  • [22] ANTIGENIC PROFILE OF HUMAN-LUNG INTERSTITIAL AND ALVEOLAR MACROPHAGES
    KOBZIK, L
    HANCOCK, W
    OHARA, C
    TODD, R
    GODLESKI, J
    LABORATORY INVESTIGATION, 1986, 54 (01) : A32 - A32
  • [23] In Vivo Characterization of Alveolar and Interstitial Lung Macrophages in Rhesus Macaques: Implications for Understanding Lung Disease in Humans
    Cai, Yanhui
    Sugimoto, Chie
    Arainga, Mariluz
    Alvarez, Xavier
    Didier, Elizabeth S.
    Kuroda, Marcelo J.
    JOURNAL OF IMMUNOLOGY, 2014, 192 (06): : 2821 - 2829
  • [24] ALVEOLAR MACROPHAGES .2. INHIBITION OF LYMPHOCYTE-PROLIFERATION BY PURIFIED MACROPHAGES FROM RAT LUNG
    HOLT, PG
    IMMUNOLOGY, 1979, 37 (02) : 429 - 436
  • [25] Lead nitrate induced apoptosis in alveolar macrophages from rat lung
    Shabani, A
    Rabbani, A
    TOXICOLOGY, 2000, 149 (2-3) : 109 - 114
  • [27] Broncho Alveolar Dendritic Cells and Macrophages Are Highly Similar to Their Interstitial Counterparts
    Maisonnasse, Pauline
    Bordet, Elise
    Bouguyon, Edwige
    Bertho, Nicolas
    PLoS One, 2016, 11 (12):
  • [28] ISOLATION AND CHARACTERIZATION OF MACROPHAGES FROM RAT EMBRYONIC MUSCLE CULTURE
    HONDA, H
    KIMURA, H
    ROSTAMI, A
    JOURNAL OF LEUKOCYTE BIOLOGY, 1992, 52 (05) : 537 - 544
  • [29] ABNORMALITIES IN THE PROCOAGULANT ACTIVITY OF ALVEOLAR MACROPHAGES FROM PATIENTS WITH INTERSTITIAL LUNG-DISEASE
    CHAPMAN, HA
    STONE, OL
    ALLEN, CL
    CHEST, 1986, 89 (03) : S185 - S187
  • [30] Alveolar macrophages drive lung metastasis in cooperation with interstitial macrophages by generating leukotriene B4
    Nosaka, Takuto
    Baba, Tomohisa
    Tanabe, Yamato
    Sasaki, Soichiro
    Arita, Makoto
    Nakamoto, Yasunari
    Mukaida, Naofumi
    CANCER SCIENCE, 2018, 109 : 359 - 359